Prediction of the three-dimensional structure for ribosomal protein L25

Prediction of Protein Three-Dimensional Structure

Prediction of the three-dimensional structure of Escherichia coli 30S ribosomal subunit: a molecular mechanics approach.

We introduce a computer-assisted procedure for folding large RNA chains into three-dimensional conformations consistent with their secondary structure and other known experimental constraints. The RNA chain is modeled using pseudoatoms at different levels of detail--from a single pseudoatom per helix to a single pseudoatom for each nucleotide. A stepwise procedure is used, starting with a simpl...

Protein Three Dimensional Structure Prediction on Object Oriented Database

We de ned the protein inverse folding problem with an object oriented database and an empirical hydrophobic penalty function, which was derived from the number of residues around each residue in a protein three dimensional structure. Under the database management system, we compiled the known structures of proteins and the evaluation function into one functional database. In order to compare ou...

Matching the crystallographic structure of ribosomal protein S7 to a three-dimensional model of the 16S ribosomal RNA.

Two recently published but independently derived structures, namely the X-ray crystallographic structure of ribosomal protein S7 and the "binding pocket" for this protein in a three-dimensional model of the 16S rRNA, have been correlated with one another. The known rRNA-protein interactions for S7 include a minimum binding site, a number of footprint sites, and two RNA-protein crosslink sites o...

Molecular Characterization of the Ribosomal Protein L25 from the Stem Rust Fungus

Lin, K. C., Bushnell, W. R., and Szabo, L. J. 2004. Molecular characterization of the ribosomal protein L25 from the stem rust fungus. Plant Pathol. Bull. 13: 251-260. A cDNA clone (pCRL130) encoding a 25S rRNA-binding protein (L25) was isolated from the oat stem rust fungus, Puccinia graminis f. sp. avenae. The cDNA clone contains a 660 bp insert which encodes a putative polypeptide of 158 ami...